attofluor cell chamber (Thermo Fisher)

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Thermo Fisher attofluor cell chamber
Attofluor Cell Chamber, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/attofluor+cell+chamber/Mr%2E+Frosty+Freezing+Container/pm41831447-140-18-14
Average 97 stars, based on 1 article reviews

attofluor cell chamber - by Bioz Stars, 2026-09

97/100 stars

Images

Microscopy:

Article Title: MitoTracker transfers from astrocytes to neurons independently of mitochondria.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines Primary murine cortical astrocytes derived from GFAP-cre x MitoTag mice, postnatal day 0–2 Generated by the authors of this paper N/A Primary murine cortical astrocytes derived from C57BL/6J mice, postnatal day 0–2 Generated by the authors of this paper N/A Primary cortical neurons derived from C57BL/6J mice, embryonic day 16.5 Generated by the authors of this paper N/A Experimental models: Organisms/strains Mouse: C57BL/6J The Jackson Laboratory 000664; RRID:IMSR_JAX:000664 Mouse: B6.Cg-Tg(Gfap-cre)77.6Mvs/2J The Jackson Laboratory 024098; RRID:IMSR_JAX:024098 Mouse: B6N.Cg-Gt(ROSA)26Sor tm1(CAG− EGFP * )Thm /J The Jackson Laboratory 032675; RRID:IMSR_JAX:032675 Recombinant DNA pAAV2/1 James M. Wilson Addgene plasmid #112862; RRID:Addgene_112862 pDsRed-Mito-7 Michael Davidson Addgene plasmid #55838; RRID:Addgene_55838 pCAG mito-mTagBFP2 Franck Polleux Addgene plasmid #105011; RRID:Addgene_105011 pZac2.1-GfaABC1D-TurboID(full)-HA-GPI Scott Soderling Addgene plasmid # 166055; RRID:Addgene_166055 Software and algorithms Fiji (2.16.0) Schindelin et al. 29 https://imagej.net/software/fiji/ Microvolution® Microvolution https://www.microvolution.com/ Prism 10.4.1. .. GraphPad https://www.graphpad.com/ Micro-Manager v2.1.0 Edelstein et al. 30 https://micro-manager.org/ Other Mr Frosty Freezing Container Thermo Scientific Cat #5100-0001 Attofluor Cell Chamber, for microscopy Invitrogen Cat #A7816 Confocal microscope Visitech International VT-iSIM e2 Cell Reports Methods 6, 101338, March 23, 2026 OPEN ACCESS with 0.5mg/mL PLL (diluted in dH 2 O) at 37 ◦ C. The following day, coverslips were washed twice in dH2O and left in attachment media (10% heat inactivated horse serum, 1mM sodium pyruvate, 33mM Glucose in MEM) at 37 ◦ C, 5% CO2 until use. ..

Article Title: Synergistic regulatory mechanisms in glycolysis revealed by pathway transplantation
Article Snippet: .. Prior to microscopy, cells were transferred to an Attofluor cell chamber (Thermo Fisher) containing a Concanavalin A precoated coverslip, prepared as described in reference 85, and incubated at 30°C for 30 min. .. Cells were imaged at 30°C using a Nikon Ti Eclipse widefield fluorescence microscope (Nikon, Tokyo, Japan) with an Andor Zyla 5.5 sCMOS camera (Andor) and a SOLA 6-LCR-SB power source (Lumen cor, Beaverton, USA).

Article Title: Passage of the HIV capsid cracks the nuclear pore.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Fiji 2.1.0/1.53c Schindelin et al.55 https://doi.org/ 10.1038/nmeth.2019 RRID:SCR_002285 SerialEM 3.7.9 and 4.0.20 Mastronarde56 https://doi.org/ 10.1016/j.jsb.2005.07.007 RRID:SCR_017293 IMOD 4.9.4 and 4.11.5 Kremer et al.57 https://doi.org/ 10.1006/jsbi.1996.0013 RRID:SCR_003297 Icy 2.0.3.0 de Chaumont et al.58 https:// doi.org/10.1038/nmeth.2075 RRID:SCR_010587 ec-CLEM (Icy plugin) 1.0.1.5 Paul-Gilloteaux et al.59 https:// doi.org/10.1038/nmeth.4170 N/A Amira 2019.3 Thermo Fisher Scientific RRID:SCR_007353 Prism 9.5.0 GraphPad Software Inc. RRID:SCR_002798 napari: a multi-dimensional image viewer for Python [software] napari contributors60 https://doi. org/10.5281/zenodo.13859720 RRID:SCR_022765 UCSF ChimeraX 1.8 Goddard et al.61 https://doi.org/ 10.1002/pro.3235 RRID:SCR_015872 Artiax (ChimeraX plugin) 0.4 Ermel et al.62 https://doi.org/ 10.1002/pro.4472 N/A Relion 3.1 Zivanov et al.63 https://doi.org/ 10.7554/eLife.42166 RRID:SCR_016274 novaCTF Turo nová et al.64 https://doi.org/ 10.1016/j.jsb.2017.07.007 N/A Fourier3D Turo nová65 https://doi.org/10.5281/ zenodo.3973621 N/A novaSTA Turo nová66 https://doi.org/ 10.5281/zenodo.5921012. .. N/A AreTomo 1.33 Zheng et al.67 https://doi.org/ 10.1016/j.yjsbx.2022.100068 N/A GAPStop(TM) Turo nová68 https://doi.org/10.5281/ zenodo.10822455 N/A cryoCAT Turo nová50 https://doi.org/10.5281/ zenodo.13916774 N/A SWISS-MODEL Waterhouse et al.69 https://doi.org/ 10.1093/nar/gky427 RRID:SCR_018123 LAMMPS Plimpton et al.70 https://doi.org/ 10.1006/jcph.1995.1039 N/A GROMACS Abraham et al.71 https://doi.org/ 10.1016/j.softx.2015.06.001 RRID:SCR_014565 VMD Humphrey et al.45 https://doi.org/ 10.1016/0263-7855(96)00018-5 RRID:SCR_001820 Other Sapphire discs, 3 ✕ 0.05 mm Wohlwend GmbH, Switzerland Cat#:405 Specimen carrier, aluminum 3 ✕ 0.5 mm, Flat / Cavity 0.3 mm Wohlwend GmbH, Switzerland Cat#:242 Specimen carrier, aluminum 3 ✕ 0.5 mm, Cavity 0.1 / 0.2 mm Wohlwend GmbH, Switzerland Cat#:241 EM grids, copper, 2 ✕ 1 mm slot, formvar support Electron Microscopy Sciences Cat:FF2010-CU Attofluor Cell Chamber Thermo Fisher Scientific Cat#:A7816 TetraSpeck Microspheres, 0.1 mm Thermo Fisher Scientific Cat#:T7279 Nikon Ti Perkin Elmer Ultra VIEW VoX 3D Perkin Elmer N/A 775 nm Expert Line STED system Abberior Instruments GmbH N/A Olympus IX83 microscope EVIDENT N/A (Continued on next page) ll OPEN ACCESS Cell 188, 1–14.e1–e11, February 20, 2025 e3 Please cite this article in press as: Kreysing et al., Passage of the HIV capsid cracks the nuclear pore, Cell (2025), https://doi.org/10.1016/ j.cell.2024.12.008 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Olympus UPlanSApo 1003 oil immersion objective (NA 1.4) EVIDENT N/A EM AFS2 freeze-substitution device Leica Microsystems N/A HPM010 high pressure freezer BAL-TEC N/A EM UC7 ultramicrotome Leica Microsystems N/A PerkinElmer UltraVIEW VoX 3D spinning-disc confocal microscope Perkin Elmer N/A Tecnai TF20 electron microscope FEI N/A Eagle CCD camera FEI N/A Quantifoil EM gold grids, 200-mesh, R 2/2 holey carbon film Quantifoil N/A EM GP2 Automatic Plunge Freezer Leica Microsystems N/A Aquilos 2 cryo-FIB-SEM microscope Thermo Fisher Scientific N/A Titan Krios G4 cryo electron microscope Thermo Fisher Scientific N/A Falcon 4 direct electron detector Thermo Fisher Scientific N/A Selectris X imaging filter Thermo Fisher Scientific N/A ll OPEN ACCESS Please cite this article in press as: Kreysing et al., Passage of the HIV capsid cracks the nuclear pore, Cell (2025), https://doi.org/10.1016/ j.cell.2024.12.008 Article

Article Title: Neurobeachin regulates receptor downscaling at GABAergic inhibitory synapses in a protein kinase A-dependent manner.
Article Snippet: .. The coverslipswere placed in an Attofluor cell chamber for microscopy (Thermo Fisher Scientific, Cat. No. A-7816) filled with Timelapse buffer (10mM HEPES, 135mM NaCl, 5mM KCl, 2 mM CaCl2, 2mM MgCl2, 15mM glucose, pH 7.4). .. A Niko microscope equipped with a spinning disk (Yokogawa, Visitron systems), solid-state lasers (488, 561, 647 and 407), objectives (60×), two EM-CCD cameras (Hamamatsu Photonics 512/1024×) and an incubation chamber for a controlled cell culture environment (5% CO2 at 37 °C) were used to acquire the images.

Imaging:

Article Title: The bat influenza A virus subtype H18N11 induces nanoscale MHCII clustering upon host cell attachment
Article Snippet: .. Glass cover slips were mounted in an AttoFluor cell chamber (ThermoFisher), for imaging. .. PALM was performed on a Nikon Ti Eclipse NSTORM microscope equipped with four laser lines at 405 nm (Coherent), 488 nm (Sapphire, Coherent), 561 nm (Sapphire, Coherent) and 642 nm laser (F-04306-113, MPB Communications).

Incubation:

Electron Microscopy:

other:

Article Title: Passage of the HIV capsid cracks the nuclear pore.
Article Snippet: Unbound fiducials were washed off on several drops of water and grids were transferred on 25mmglass coverslips mounted in a water-filled ring holder for microscopy (Attofluor cell chamber, Thermo Fisher Scientific).

Review